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KMID : 0390220000110020261
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Journal of Clinical Otolaryngology, Head and Neck Surgery
2000 Volume.11 No. 2 p.261 ~ p.266
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Ecpression of ICAM-1 by IFN-γ and IL-4 in Cultured Normal Human Nasal Epitelial Cells
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김용대/Yong Dae Kim
박준철/이준혁/조중석/이재억/배창훈/송시연/윤석근/Jun Chul Park/Joon Hyuk Lee/Jung Suck Cho/Jae Uk Lee/Chang Hoon Bai/Si Youn Song/Seok Keun Yoon
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Abstract
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Background and Objectives : Intercellular adhesion molecule-1(ICAM-1) is an important molecule in immune
and inflammatory responses. It is regulated by IL-1β, IL-4, TNF-α, and IFN-γ on many cell types. The aim of
this study was to investigate the effects of INF-γ and IL-4 on expression of ICAM-1 in cultured normal human
nasal epithelial (NHNE) cells. Materials and Methods : NHNE cells were prepared by primary culture method
of monolayer culture of dissociated cells from inferior turbinate mucosa. Primary cultured cells were
characterized as an epithlial cell type by immunofluorescence assay and transmission electron microscopy. We
analyzed the quantitative expression of ICAM-1 on cultured NHNE cells by treated with IFN-γ AND IL-4 using
fluorescence activated cell sorter (FACS). Results : Treatment of cultured NHNE cells with IFN-γ(1ng/ml) for
24 hours induced 4-fold increase of the surface ICAM-1 showed no effect even though the concentration of it was
intensity fluorescence (MIF). However, IL-4 showed no effect even though the concentration of it was
increased gradually. INF-γ induced ICAM-1 expression accordind to the time course was significantly highest
level at 24 hours. There were no significant changes in expression of ICAM-1 by IL-4 according to the time
course. Conclusion : These finding suggest that IFN-γ is a potent ICAM-1 inducer, but IL-4 have no effects
on expression of ICAM-1 on cultured NHNE cells.
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KEYWORD
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